Table 3.

Rescue of bmp4 expression pattern by hyaluronidase treatment

Genotype	Injection	Broad bmp4	Concentrated bmp4
wild-type	PBS	-	11/11 (100%)
Ztmem2	PBS	22/22 (100%)	-
wild-type	Hyaluronidase	5/58 (9%)	53/58 (91%)
Ztmem2	Hyaluronidase	4/22 (18%)	18/22 (82%)

Summary of experiments evaluating whether injection of hylauronidase can rescue the bmp4 expression pattern in Ztmem2 mutants. Phenotypes were assessed as in Table 1, although some examples of “broad bmp4” in the hyaluronidase-injected wild-type embryos were not strictly Ztmem2-like in their appearance and instead simply exhibited abnormally broad bmp4 expression. Hyaluronidase treatment is able to efficiently rescue the bmp4 expression pattern in Ztmem2 mutants (as in Fig. 8D). Significant difference between hyaluronidase-injected and PBS-injected Ztmem2 mutants was determined using Fisher’s exact test (p<0.001).