Table 4.

Rescue of Wnt signaling distribution by hyaluronidase treatment

Genotype	Injection	Expanded Wnt activity	Restricted Wnt activity
wild-type	PBS	-	5/5 (100%)
Ztmem2	PBS	4/4 (100%)	-
wild-type	Hyaluronidase	4/25 (16%)	21/25 (84%)
Ztmem2	Hyaluronidase	4/16 (25%)	12/16 (75%)

Summary of experiments evaluating whether injection of hylauronidase can rescue the distribution of Tg(7xTCF-Xla.Siam:GFP) reporter activity in Ztmem2 mutants. Data indicate the fraction of embryos displaying expanded Wnt signaling activity (as in Fig. 9D) or restricted Wnt signaling activity (as in Fig. 9B,F,H). We note that some examples of expanded Wnt activity among the hyaluronidase-injected wild-type embryos were not strictly Ztmem2-like in their appearance and instead simply exhibited abnormal expansion of Wnt activity outside of the AVC. Hyaluronidase treatment is able to restrict the distribution of Wnt signaling in Ztmem2 mutants. Significant difference between hyaluronidase-injected and PBS-injected Ztmem2 mutants was determined using Fisher’s exact test (p=0.01).