Table 5.

Rescue of bmp4 expression pattern by increased levels of tmem2 and variants

Genotype	Injected mRNA	Broad bmp4	Concentrated bmp4
wild-type	uninjected	-	78/78 (100%)
Ztmem2	uninjected	28/28 (100%)	-
wild-type	300 pg tmem2	-	44/44 (100%)
Ztmem2	300 pg tmem2	3/14 (21%)	11/14 (79%)
wild-type	300 pg sc-tmem2	-	77/77 (100%)
Ztmem2	300 pg sc-tmem2	16/21 (76%)	5/21 (24%)
wild-type	300 pg R267H	-	36/36 (100%)
Ztmem2	300 pg R267H	4/12 (33%)	8/12 (67%)

Summary of experiments evaluating whether increased expression of tmem2 or tmem2 variants can improve rescue of the bmp4 expression pattern in Ztmem2 mutants. Phenotypes of wild-type sibling or Ztmem2 mutant embryos were assessed as in Table 1. For both full-length tmem2 and sc-tmem2, the degree of rescue provided by injection of 300 pg of mRNA is similar to that provided by 150 pg of mRNA (compare to Table 1). In contrast, doubling the dosage of R267H mRNA significantly improves its efficacy of rescue (compare to Table 1). Significant difference between the effectiveness of 150 and 300 pg dosages was determined using Fisher’s exact test (p<0.001 for R267H). Other analyses with Fisher’s exact test did not yield significant differences: 150 pg tmem2 vs. 300 pg tmem2, p>0.1; 150 pg sc-tmem2 vs. 300 pg sc-tmem2, p=1; 300 pg tmem2 vs. 300 pg R267H, p>0.1.