Figure 2.

(A) Karyotypes of the parental cell line LS174T exhibited a mixture of near-diploid (n = 45, left), near-diploid with trisomy of chromosome 7 (n = 46, middle) and near-tetraploid cells (n = 90, right). (B) Left, karyotypes of near-diploid clones C4 and C10 (n = 45) and near-diploid clones C2 and C13 with trisomy of chr7 (n = 46); middle, near-tetraploid clones C5, C6, C7 and C8; right, clones with mixture of karyotypes C9 and C12. (C) Karyotypes of LS174T clones C8 and C11. At time 0 (left), C8 cells were pure near-tetraploids, and C11 cells were almost pure near-diploids. After 60 days of culture (right), C8 cells remained mostly near-tetraploid with the occasional appearance of higher ploidy cells, while C11 cells exhibited 13.3% of near-tetraploid cells. (D) Metaphases of the karyotypes presented in panel C.