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. 2021 Oct 20;13(21):5268. doi: 10.3390/cancers13215268

Figure 4.

Figure 4

Sensitivity to viruses and virus replication of DBTRG-05MG and their derivatives with overexpression or shRNA-mediated silencing of genes for VSV-I and enteroviruses. The sensitivity and replication were measured in quadruplicates for a range of IFNα concentrations by virus titration in the sensitive RD cells. Cell cultures were treated with IFNα for 24 h, followed by infection with a virus. The tissue culture infectious dose required to reach the fifty percent endpoint, lgTCID50/mL, was measured 24 h after infection. Labels: ↑↓ denote a significant increase or decrease in cell sensitivity/virus replication with t-test p-value < 0.05, * significance corrected for multiple comparisons using the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli, Q-value < 0.05 (GraphPad Prism). Abbreviations: VSV, Vesicular stomatitis virus Indiana strain; PV3, Sabin vaccine strain of type 3 poliovirus; NDV, Newcastle disease virus strain H2; CB5, Coxsackie B5 virus strain LEV14; CA7, Coxsackie A7 strain LEV8; E12, Echovirus 12 strain LEV7.