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. 2021 Oct 31;13(21):5482. doi: 10.3390/cancers13215482

Figure 1.

Figure 1

Characterization of exosomes derived from normal and lung cancer cell lines. (a) Average size of SEC fractions 6–8 determined by NTA. Average sizes of SEC fractions 6, 7, and 8 were 134.131 ± 1.99, 138.73 ± 6.98, and 131 ± 3.2 nm (mean ± SD), respectively. (b) Western blot analysis of the CD63, CD9, and CD81 exosomal proteins in the exosome lysates (6 µg of total protein/well of acrylamide gel). (c) TEM analysis shows the typical cup shape of the exosomes. Scale bar = 100 nm. (d) Average size of SEC fractions 6–8 from healthy and patient plasmas determined by NTA were 135.36 ± 5.31 and 138.71 ± 12.40 (mean ± SD), respectively. (e) Western blot analysis of the CD63 and CD9 exosomal proteins in the exosomal lysates. (f) TEM analysis shows the typical cup shape of the exosomes derived from plasma. Scale bar = 100 nm. (g) TEM analysis shows immunogold labeling using an anti-CD63 antibody for the exosomes isolated from HPAEpiC and human plasmas. Scale bar = 100 nm.