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. 2021 Oct 28;13(21):5408. doi: 10.3390/cancers13215408

Figure 1.

Figure 1

Expression of SIX1 and its co-activator EYA2 in colorectal cancer cell lines in normal culture conditions and after incubation with TGF-β. (A) Comparison of SIX1 expression in colon circulating tumor cell (CTC) lines (pooled data), and in primary (HT-29) and metastatic (SW620) colorectal cancer cell lines (microarray data). (B) RT-qPCR analysis of SIX1 expression in each of the nine CTC lines and in SW620 cells. (C) RT-qPCR analysis of SIX1 expression in cells incubated or not with TGF-β. (D) EYA2 expression profile in cells incubated or not with TGF-β. a: p-value = 2.73 × 10−9; b: p-value = 9.80 × 10−11. All the RT-qPCR results were normalized to the B2M expression level in each sample; * p < 0.05, *** p < 0.001, ns: not significan.