Increased MYC and EZR gene expression in the nine CTC lines compared with HT-29 cells. (A) Comparison of MYC expression in CTC lines, HT-29, and SW620 cells (microarray data). (B) RT-qPCR analysis of MYC expression in each CTC line and in HT-29 and SW620 cells. (C) Comparison of EZR expression in CTC lines, HT-29, and SW620 cells (microarray data). (D) EZR expression level in each CTC line, HT-29, and SW620 cells. (E) Immunofluorescence analysis of ezrin expression in the nine CTC lines. c: p-value = 1.00 × 10−4; d: p-value = 6.41 × 10−7. All RT-qPCR results were normalized to the B2M expression level in each sample; * p < 0.05, ** p < 0.01, *** p < 0.001.