SLUG and TAL1-PP22 overexpression respectively reduce GSC growth. (A) Cell counts of Gb4 and Gb7 cells 5 days after transduction of Control, SLUG or TAL1-PP22-overexpressing lentiviruses. Data is shown as a % of the control condition and is representative of 3 independent experiments, n = 5 wells per conditions. Statistical analyses using rank Mann–Whitney tests, **, p < 0.01. (B) WB analyses of Gb4 and Gb7 cells after SLUG or TAL1-PP22 overexpression, showing no mutual modulation of expression and no significant change in OLIG2 and SOX2 expression (n = 1). Control for overexpression of SLUG and expression of SOX2 was normalized to β-actin; control for TAL1-PP22 overexpression and expression of OLIG2 was normalized to GAPDH.