Table 1.
Measured GSH and GSSG Levels in Rat Liver Cytosol and Mitochondria
| age/fraction treatment | GSH (nmol/mg) | GSSG (nmol/mg) | GSH:GSSG | |
|---|---|---|---|---|
| young cytosol | DCA-treated control | 82.0 ± 17.1**** | 1.85 ± 0.59 | 48.7 ± 15.1**** |
| 79.9 ± 18.9**** | 2.05 ± 0.54 | 40.5 ± 11.2* | ||
| adult cytosol | DCA-treated control | 47.9 ± 12.5 | 2.53 ± 0.36 | 19.3 ± 5.9 |
| 57.4 ± 11.5 | 2.40 ± 0.44 | 24.9 ± 7.6 | ||
| young mitochondria | DCA-treated control | 4.31 ± 1.25* | 0.40 ± 0.09* | 10.5 ± 2.6**** |
| 4.34 ± 1.18* | 0.39 ± 0.10* | 11.1 ± 1.4**** | ||
| adult mitochondria | DCA-treated control | 3.74 ± 1.29 | 1.01 ± 0.29 | 3.9 ± 1.3 |
| 3.84 ± 0.89 | 0.94 ± 0.25 | 4.3 ± 1.6 | ||
| young whole liver | DCA-treated control | 8.63 ± 2.97**** | 0.23 ± 0.11* | 44.9 ± 25.8 |
| 8.17 ± 3.04**** | 0.19 ± 0.07* | 51.0 ± 34.3 | ||
| adult whole liver | DCA-treated control | 3.91 ± 0.63 | 0.08 ± 0.02 | 52.6 ± 13.2 |
| 3.95 ± 0.64 | 0.08 ± 0.02 | 51.9 ± 11.1 | ||
a
Values presented are mean ± SD, n = 44 for young rat DCA-treated measurements, n = 42 for young rat control measurements, n = 48 for adult rat DCA-treated measurements, and n = 40 for adult rat control measurements. The detailed time-point data that are summarized in Table 1 are shown in Figure 7. The data were analyzed to test for age-related differences between fraction treatments by Student’s t-test,
*
p < 0.05,
****
p <0.0001.