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. 2021 Nov 5;13(21):5561. doi: 10.3390/cancers13215561

Figure 1.

Figure 1

Example of images of γH2AX clusters after 180 min after 2 Gy irradiation of human skin fibroblasts. (a) Clusters detected by the original program used in [21]. (b) Cluster image obtained with the DBSCAN algorithm (cluster parameter values: Nmin = 110; ε = 200 nm). (c) The same image as (b) but with the closing function applied. The high co-incidence of (a) and (c) can be observed. Scale bar: 2 µm.