Figure 4.

Example of the three 1st-generation heatmaps of γH2AX clusters compared with each other for the control group of the MCF-7 cell line (10 min values). (a) The cluster similarities of the components (dim 0), (b) those of the holes (dim 1), and (c) the average values for each of the pixels from (a) and (b) are presented. Note: This example is shown because the differences in the similarity of components and holes is large. In the dim 0 heatmap, many clusters have a similarity of above 0.98 and the lowest values are around 0.96. In contrast, in the dim 1 heatmap, the highest value is at about 0.6 and practically all values are in the range of 0.2 to 0.6, excluding the diagonal ones (identity). Therefore, the average is dominated by the dim 1 values. Moreover, the problematic effect of the diagonal can be seen quite effectively as the range of values can only be observed up to a certain degree of accuracy. The differences between the colors for a relatively large spectrum of values are hardly recognizable.