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. 2021 Nov 3;13(21):5516. doi: 10.3390/cancers13215516

Figure 6.

Figure 6

Transcriptome-wide mRNA expression profiling revealed that TP-472 treatment leads to upregulation of pro-apoptosis genes in melanoma cells. (A). Reactome-based functional pathways analysis revealed upregulation of several genes associated with the p53 pathway and apoptosis. (B). Heatmap showing the several pro-apoptosis genes that were upregulated after 5 μM, or 10 μM of TP-472 treatment. (C). A375 cell line was treated with 10 μM of TP-472 for 24 h. Immunoblotting was performed to measure the protein expression of the shown candidates. ACTINB were measured as loading controls. (D). Bar diagram showing apoptosis of melanoma cells treated with 10 μM TP-472 for 48 h (percentage apoptosis in inhibitor-treated cells relative to vehicle-treated control cells). (E). Model showing that TP-472 treatment of melanoma cells leads to suppression of ECM-mediated pro-oncogenic signaling pathways and activation of apoptosis genes, ultimately leading to inhibition of melanoma cell growth and proliferation. Data represent the mean ± SEM. ns = not significant, ** p < 0.01, *** p < 0.001.