Figure 2.

IR downregulated miR–541–5p to promote EMT. (A) Real-time PCR analysis verified the transfection efficiency of the miR–541–5p inhibitor (50 nm) in A549 cells. Detection of EMT-related protein changes after 48 h transfection by WB. The bar graph on the right shows the quantitative analysis of the protein using ImageJ. (B) Real-time PCR verified the transfection efficiency of the miR–541–5p mimic (50 nm) in A549 cells. Right: Re–overexpression of miR–541–5p after irradiation to observe the changes in the EMT-related proteins and the quantitative analysis of these proteins. Real-time PCR verified the transfection efficiency of the miR–541–5p inhibitor (C)/mimic (D) in Beas 2B cells. Detection of EMT–related protein changes after 48 h transfection by WB. The bar graph on the right shows the quantitative analysis of the protein using ImageJ. The bar graphs show the gray value analysis. * p < 0.05 versus the control; # p < 0.05 versus IR. ** p < 0.01, *** p < 0.001