Figure 5.

Structural and phylogenetic analysis of dsrm domains. (A) Maximum likelihood analysis including all domains with similar structure to dsrm present in the proteins DCR1, DCR2, DROSHA, LOQS, PASHA and R2D2 from species belonging to the five insect orders (Coleoptera, Diptera, Hemiptera, Hymenoptera and Lepidoptera). Dicer Dimer and Staufen C domains were inserted on this analysis due to have high structural similarity with dsrm. Each triangle represents an insect order, according to the colour legend presented, and it is proportional to the number of branches present. The outgroup (hidden) used was the dsrm domain from human DROSHA (PDB ID: 5B16) and the bootstrap values are represented by dark blue circles (minimum 70). (B) Structural model of dsrm domain from human DROSHA (PDB ID: 5B16, B), interacting with RNA molecule, and (C) the same domain from human DROSHA superimposed with a Dicer Dimer from Arabidopsis thaliana DCL protein (PDB ID: 2KOU), highlighting the differences and similarities between these two domains. (D and E) Superposition of the models from LOQS dsrm-II and DCR2 Dicer Dimer domains, representing dsrm domains that hypothetically can interact preferentially with dsRNAs and proteins, respectively. In (D), the species that represented each insect order were: Coleoptera: T. castaneum (TC011666); Diptera: D. melanogaster (FBpp0080075); Hemiptera: B. tabaci (Bta01704); Hymenoptera: A. melífera (GB47214); and Lepidoptera: M. sexta (Msex2.00134). In (E), the species that represented each insect order were: Coleoptera: T. castaneum (TC001108); Diptera: D. melanogaster (FBpp0086061); Hemiptera: B. tabaci (Bta10685); Hymenoptera: A. melífera (GB48923); and Lepidoptera: M. sexta (Msex2.04462). In both (D) and (E) were highlighted the main variability spots