TABLE 1.
Detection of Campylobacter species
| Source | No. of samples examined | No. of samples positive by specific technique/total no. of samples positive by both techniquesa
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
|
C. jejuni-C. coli
|
C. upsaliensis
|
C. hyointestinalis
|
C. lari
|
||||||
| PCR-ELISA | Culture | PCR-ELISA | Culture | PCR-ELISA | Culture | PCR-ELISA | Culture | ||
| Lab A | 1,107 | 106/121 | 108/121 | 3b/3 | 1c/3 | 1/1 | 0/1 | ||
| Lab B | 725 | 63/73 | 65/73 | 1/1 | 0/1 | 1/1 | 1c/1 | ||
| Lab C | 1,178 | 186/203 | 189/203 | 5/5 | 0/5 | 1/1 | 0/1 | ||
| Lab D | 300 | 55/57 | 35/57 | 1/1 | 0/1 | 1/1 | 0/1 | ||
| Lab E | 166 | 20/21 | 12/21 | ||||||
| Lab F | 162 | 31/36 | 35/36 | 1/1 | 1c/1 | ||||
| Lab G | 100 | 17/18 | 17/18 | ||||||
| Total | 3,738 | 478/529 | 461/529 | 11/11 | 2/11 | 3/3 | 1/3 | 1/1 | 0/1 |
a
For example, in the case of C. jejuni-C. coli, a total of 461 of 3,738 isolates were detected by culture, while 478 of 529 were identified by the PCR algorithm described in Fig. 1 (PCR-ELISA). A total of 529 were found by a combination of methods; i.e., 68 were not detected by culture and 51 were not identified by PCR-ELISA.
b
Includes one sample with a mixed infection with C. upsaliensis and C. jejuni detected by PCR-ELISA.
c
For these samples, culture identified the strain only as a “Campylobacter sp.” Subsequent species identifications were by PCR-ELISA.