Table 3.

Summary of identified miRNAs in molluscs

Species	miRNA no.	Library source	Sequencing technology or method	Function	Reference
C. farreri	68	Haemocytes	Illumina Genome Analyser	Immune response	[103]
C. gigas	100	Different developmental stages and tissues	Illumina HiSeq 2000	Biological process	[25]
C. gigas	199	Haemocytes	Illumina HiSeq 2000	Immune response	[74]
C. gigas	202	Gill	Illumina HiSeq 2000	Osmotic stress response	[81]
C. gigas	331	Haemocytes	Ion Torrent Proton sequencer	Immune response	[104]
C. hongkongensis	87	Gill	Illumina HiSeq 2000	Osmotic stress response	[81]
C. hongkongensis	113	Testis and ovary	Illumina HiSeq 2000	Gonad differentiation	[105]
Hyriopsis cumingii	170	Mantle exosome	Illumina HiSeq 4000	Nacre colour formation	[106]
Littorina littorea	7	Hepatopancreas and foot muscle	RT-PCR	Extreme Environment stress	[87]
M. galloprovincialis	137	Soft tissues	BGISEQ-500 sequencing	Biological processes	[107]
Ostrea edulis	120a	Haemocytes	Commercial microarray	Immune response	[73]
Patella vulgata	45b	Male gonad	Illumina HiSeq 2000	microRNA evolution	[59]
P. fucata	228	Adductor muscle, gill, mantle and gonad	IonTorrent Proton Sequencer	Biomineralization process	[26]
P.martensii	258	Mixed tissues library	Solexa deep sequencing platform	Biomineralization understanding	[108]
P. martensii	30b	Pearl sac	Illumina Hiseq2000	Biomineralization understanding	[60]
T. granosa	254	Haemocytes	Illumina HiSeq 2000	Heavy metal stress response	[46]
A.californica	170	Isolated CNS and whole body with CNS removed	454 sequencing platform	Long-term plasticity of synapses in Aplysia mature nervous system	[79]
Haliotis midae	40	Mixed tissues library	Illumina Genome Analyser II and 454 GS FLX platform	Immune and stress response	[109]
H. rufescens	53	Foot, and visceral mass+larvae	454 sequencing platform	miRNA evolution	[13]
L. gigantea	63	-	miRBase 22.0	miRNA evolution	[10]
Lymnaea stagnalis	385	Central nervous system	Illumina GAIIx platform	Neuronal regeneration	[89]
Otala lactea	75 c	Foot muscle	RT-PCR	Estivation responsive	[88]
Dosidicus gigas	39 c	Brain, mantle muscle, and branchial heart	RT-PCR	Hypoxia-induced metabolic suppression	[84]

a: Differently expressed miRNAs; b: Computationally predicted miRNA; c: Conserved miRNAs from invertebrates.