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Immunofluorescent images from patient no. 1 demonstrating different CTC phenotypes. Images obtained on a Leica DM6000B microscope at 40× magnification. Top row-an EpCAM+ CD45− (DAPI+) epithelial CTC is labelled next to two EpCAM- CD45+ leukocytes. Bottom row–a CD45- EpCAM+ cell also with low expression of N-cadherin, indicating the process of a transition from an epithelial to mesenchymal (EMT) phenotype.
