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. 2021 Nov 5;13(21):5551. doi: 10.3390/cancers13215551

Figure 1.

Figure 1

Validation and epigenetic characterization of the H3.3K27M-engineered cell models. (a). Microscopy pictures of the Res259 cell line, WT cells or cells expressing the H3F3A mutated (H3.3K27M) or unmutated (H3.3) gene. Fluorescence was acquired for the mCherry signal (top lane) or Hoechst signal (bottom lane). Scale bars 10 µm. (b). Western blot of H3K27M and H3.3 was performed on all cell lines. Other epigenetic marks were also evaluated as H3K27me3/me2/me1 and H3K27ac. H4 was used as a loading control.