Figure 1.

BC1 ncRNA is abundant in the GV oocyte with polysomal association

(A) qRT-PCR analysis of BC1 expression in the GV, MII oocytes and 2-cell embryo. Values obtained for GV stage were set as 1. Gapdh mRNA and Neat2 lncRNA were used as controls. Relative expression; mean ± SD; Student’s t-test: **p< 0.01; ***p< 0.001, ns for non-significant, n=3. (B) Expression of BC1 in the nucleus and cytoplasm of oocyte. Values obtained from qPCR of the nucleus and cytoplasm combined as 100%. Gapdh mRNA and Neat2 lncRNA were used as controls. See also Figure S1. (C) Polysomal association of RNAs coding for Gapdh, Neat2 and BC1 in the GV and MII oocyte. Expression in the GV stage was set as 1. Gapdh mRNA and Neat2 lncRNA were used as controls. Relative expression, mean ± SD; Student’s t-test: *p< 0.05; ***p< 0.001, ns for non-significant, n ≥ 2. NP, non-polysomal; P, polysomal. See Figure S2 for validation of polysomal fractionation.