Figure 4.

BC1 physically interacts with candidate mRNAs via protein interplay

(A) Scheme of BC1 anti-sense DNA 5ʹ-biotinylated oligonucleotide probes related to BC1. (B) Pull-down from whole oocytes lysate with three probes complementary to BC1 ncRNA (BC1) and without probes as a negative control (Cntr). Endogenous BC1 and Gapdh RNAs were used as additional controls. Values obtained from control were set as 1. Relative expression; mean ± SD; Student’s t-test: *p< 0.05; **p< 0.01, ***p< 0.001, ns for non-significant, n≥2. (C) Pull-down from purified total RNA with three probes complementary to BC1 ncRNA (BC1) and without probes as a negative control (Cntr). Endogenous BC1 and Gapdh RNAs were used as additional controls. Values obtained from control were set as 1. Relative expression; mean ± SD; Student’s t-test: *p< 0.05; ns for non-significant, n=3.