Figure 7.

Overexpression of BC1 does not alter oocyte or early embryo development

(A) Analysis of the maturation and developmental competence of the oocyte with BC1 overexpression. Relative number of oocytes; mean ± SD; Student’s t-test: ns for non-significant, n ≥ 42. See Figure S3 for validation of overexpression. (B) Analysis of the morphology of the MII oocyte with overexpression of BC1. Representative confocal images of MII oocytes labelled with tubulin antibodies in the control (Cntr) and OE of BC1; scale bars 25 µm; Tubulin green; DNA blue; n ≥ 20. (C) Bright-field images of blastocyst development in control and BC1 OE groups.