Figure 5.

PD-1 blockade improves the function of NKT-like cells from healthy donors

Frequency of (A) TNF-α⁺ cells in NKT-like cells and (B) IFN-γ⁺ cells in NKT-like cells from HD in the presence of anti-PD-1 mAb or isotype mAb. PBMCs from healthy donors and were stimulated with PMA (100 ng/mL) and ionomycin (1 μg/mL) for 5 hours in the presence of 20 μg/mL anti-PD-1 mAb or an equal dose of mouse IgG1, κ isotype antibody. Frequency of (C) TNF-α⁺ cells, (D) IFN-γ⁺ cells, and (E) CD107a⁺ cells in NKT-like cells from HD in the presence of anti-PD-1 mAb or isotype mAb. PBMCs from HD were stimulated with anti-CD3 (2 μg/mL)/anti-CD28 (1 μg/mL) for 6 hours in the presence of 20 μg/mL anti-PD-1 mAb or an equal dose of mouse IgG1, κ isotype antibody. P-values < 0.05 are considered statistically significant. *P < .05; **P < .01; ***P < .001.