TABLE 1.

Primer sequences and location

Primer	DNA sequence (5′–3′)a	Locationb	Species specificity
AFU2S	ATG TCT AAG TAT AAG CAA TTT A	17–38	Aspergillus
AFU2AS	CTG TTA TTG CCG CGC ACT TCC A	1366–1387	Aspergillus
AFU3S	GCG AGT ACT GGT CCG GCT GGA	628–648	Aspergillus
AFU3AS	CCA GCG GCC CGC AAA TGC GG AC	1314–1335	Aspergillus
AFU4S	TAC TTA GAC ATG CAT GGC TTA A	6–27	Aspergillus
AFU4AS	TAG AGG AAG TAA AAG TCG TAA	1704–1724	Aspergillus
AFU5S	AGG GCC AGC GAG TAC ATC ACC TTG	1436–1459	Aspergillus
AFU5AS	GG G (AG)GT CGT TGC CAA C(CT)C (CT)CC TGA	1648–1771	Aspergillus
AFU7S	CGG CCC TTA AAT AGC CCG	1296–1313	Aspergillus
AFU7AS	GA CCG GGT TTG ACC AAC TTT	1681–1700	Aspergillus
AFU8S	GTC CGC ATT TGC GGG CCG CT	1314–1333	Aspergillus
AFU8AS	TGC CAA CTC CCC TGA GCC AG	1643–1662	Aspergillus
AFU9S	GCA CGC GCG CTA CAC TGA CAG GGC	1417–1440	Universal
AFU9AS	GGC CTC ACC GAG CCA TTC AAT CGG	1613–1636	Universal
AFU10AS	GCG ACG GGC GGT GTG TAC AAA GGG	1584–1607	Universal
G6PD1S	CAG CGT CAT GGC AGA GCA GGT GGC	3344–3367	Human
G6PD1AS	GGA GAT ACT CAC CGA TGC ACC CAT	3459–3482	Human

^aNucleotides in parentheses are degenerate. 

^bFor the AFU primers, locations are from the 18S rRNA gene sequence of A. fumigatus DNA (GenBank accession no. AB008401). For the G6PD primers, locations are from the glucose-6-phosphate dehydrogenase gene sequence of human DNA (GenBank accession no. X55448).