Figure 1.

Knockdown efficiency of genes in T. castaneum triggered by 100 bp dsCYP6BQ6. (A) The number connected with a dash to the gene name represents the identity between the gene fragment and dsCYP6BQ6. The dsEGFP of the same length was used as control. Values are presented as mean±SE, n = 4 (*, p < 0.05; **, p < 0.01; ***, p < 0.001). The ‘n = 4’ represents four biological repeats, each biological repeat contains 10 larval individuals for RNAi experiments. (B) Alignments showing the complementarity between dsCYP6BQ6 and the corresponding area of the extremely sensitive or insensitive genes, CYP6BQ12, CYP6BK13, CYP6A1 and CYP6BK7, with the longest fragments of perfectly or almost perfectly matching sequence are indicated