The non-target effects in T. castaneum induced by dsRNA synthesized using Diabrotica virgifera virgifera SNF7 gene fragment as a template (dsDvSNF7). (A) Alignment of sequences of SNF7 homologs from T. castaneum and D. virgifera. (B) The expression depletion of T. castaneum SNF7 triggered by dsDvSNF7 and dsTcSNF7. (C) Mortality of T. castaneum induced by dsDvSNF7 and dsTcSNF7 (Tc, T. castaneum; Dv, D. virgifera). (D) Mortality of T. castaneum induced by dsCsEF1 and dsTcEF1. Mean±SE (n = 4) are presented. *, p < 0.05; **, p < 0.01; ***, p < 0.001)