Table 1.

Knockdown efficiency of different genes in T. castaneum triggered by dsRNA containing evenly distributed single mismatching bases

Gene name	Expression levelsa	Knockdown efficiency (%) of the dsRNA with varied matching nucleotide ratio
		6:1	5:1	4:1	3:1
CYP4Q7	3142.8	40.6 ± 3.8b	27.7±b	−5.3 ± 7.8	−42.9 ± 7.5
Drip	272.4	21.8 ± 3.1b	0.32 ± 3.9	-	-
AANAT1	245.6	37.9 ± 2.4b	0.8 ± 2.2	−15.4 ± 7.2	−37.0 ± 13.8
CYP6BK13	188.7	18.2 ± 0.6b	2.9 ± 9.5	6.0 ± 7.1	0.51 ± 7.4

^aExpression levels were calculated using CPR18 as a reference gene.

^bSignificant knockdown.