Table 3.
Comparison between sequence matching (dsRNA with target gene) and knockdown efficiency for dsRNAs inducing insignificant RNAi in T. castaneum.
| Interaction couple | Gene expression level |
dsRNA/gene identity | Longest segment |
Per cent knockdown | |
|---|---|---|---|---|---|
| Contiguous matching |
Imperfect matching |
||||
| dsCYP6BQ6/CYP6BQ9 | 6.3 | 86 | 22 | 74 | 9.2 |
| dsMutant/Drip | 272.4 | 79 | 8 | 8 | <0 |
| dsMutant/Drip | 272.4 | 80 | 10 | 15 | <0 |
| dsMutant/AANAT1 | 245.6 | 78 | 11 | 15 | 9.5 |
| dsMutant/CYP6BK13 | 188.7 | 78 | 9 | 16 | 2.9 |
| dsMutant/AANAT1 | 245.6 | 80 | 11 | 18 | <0 |
| dsMutant/CYP4G7 | 600.1 | 80 | 15 | 18 | <0 |
| dsMutant/CYP4G7 | 600.1 | 79 | 13 | 20 | <0 |
| dsMutant/CYP6BQ6 | 53.7 | 79 | 14 | 21 | 14.7 |
| dsMutant/CYP6BQ6 | 53.7 | 80 | 16 | 21 | 0.2 |
| dsMutant/CYP4G7 | 600.1 | 77 | 10 | 22 | <0 |
| dsMutant/Drip | 272.4 | 82 | 11 | 23 | 8.3 |
| dsMutant/CYP6BQ6 | 53.7 | 77 | 11 | 23 | <0 |
| dsMutant/CYP6BQ6 | 53.7 | 78 | 13 | 23 | <0 |
| dsMutant/CYP6BQ6 | 53.7 | 85 | 13 | 25 | 20.1 |
| dsMutant/AANAT1 | 245.6 | 81 | 9 | 26 | <0 |
| dsMutant/Drip | 272.4 | 83 | 10 | 26 | 14.8 |