Figure 4.

NudC specifically interacts with the cytoplasmic form of PCID2

(a) Co-immunoprecipitation from the S2 cell lysate was performed with antibodies against PCID2, NudC, or with IgG. Equal amounts of the extract (Inp) and precipitated proteins (IP) were analysed. The membrane was developed with antibodies against PCID2 or NudC.(b) The His-tagged PCID2 was purified from bacteria (lane 2) and incubated with bacterial extract containing GST-tagged NudC (lane 1). Please, note that for a better identification of the GST-tagged NudC, the extract used for incubation, was diluted (1:20). The extract was further incubated with Glutathione Sepharose, and the precipitated proteins (lane 3) were resolved on the SDS-PAGE and visualized by Coomassie staining.