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. 2021 Nov 9;10(1):2000699. doi: 10.1080/2162402X.2021.2000699

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — In vitro characterization of murine CS1-specific sdAbs. (a) Summary of ELISA, flow cytometry (on 5T33MMvv cells) and SPR of 10 selected, His₆-tagged mCS1 targeting sdAbs. (b) Flow cytometry result of His₆-tagged CS1 sdAb1 binding on 5T33vv cells (R3B23 is used as a control sdAb) (one representative result). (c) SPR sensorgram of untagged sdAb1. (d) Thermal stability of His₆-tagged, untagged and DTPA-conjugated sdAb1. (e) Amino acid sequence of 10 selected CS1 sdAbs