Figure 2.

The suppressed expression of YB-1 causes lower stability of YB-3 mRNA. Changes in stability (A-E) and synthesis (F) of some mRNAs in YB-1-null cells. (A-F) HEK293T and HEK293TΔYB-1 cells were incubated in DMEM with bromodeoxyuridine (BrU) for 24 h and then in standard medium for 0, 4, 8, or 12 h, followed by isolation of total RNA. This RNA was used for immunoprecipitation with antibodies against BrU. In the immunoprecipitate, selected mRNAs were detected by qRT-PCR. For each cell line, the mRNA amount at the 0 h point was taken to be 100%. Errors are 2 standard deviations. Two-tailed Student’s t-test was used to estimate the statistical significance. ***p < 0.001, *p < 0.05. (G) HEK293T and HEK293TΔYB-1 cells were incubated in DMEM with BrU for 24 h, followed by isolation of total RNA. This RNA was used for immunoprecipitation with antibodies against BrU. In the immunoprecipitate, selected mRNAs were detected by qRT-PCR. In HEK293T cells, the mRNA amount at the 0 h point was taken to be 100%. Errors are 2 standard deviations. Two-tailed Student’s t-test was used to estimate the statistical significance. ***p < 0.001, **p < 0.01