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. 2000 Jul;20(13):4513–4521. doi: 10.1128/mcb.20.13.4513-4521.2000

TABLE 2.

Chx inhibition of protein synthesis in the Arabidopsis cell suspension culturea

Chx concn (μM) % Incorporation of label ± SD in presence of:
Chx alone Chx + 3% sucrose
0 100 ± 8.9b
10 3.1 ± 0.29 3.3 ± 0.45
50 2.3 ± 0.10 2.3 ± 0.10
100 1.9 ± 0.16 2.3 ± 0.16
a

D7 cells were incubated for 24 h in medium without sucrose and then labeled with 35S-labeled amino acids in the presence of Chx and/or sucrose for 6 h. The label incorporated into proteins was determined for triplicate samples by TCA precipitation. Control cells supplied with sucrose alone (0 μM Chx) for 6 h incorporated 48% ± 4.3% of the total intracellular 35S label into protein. This was defined as maximal (100%) incorporation, and other results are presented as a percentage of this level. 

b

Sucrose only.