TABLE 2.
Chx inhibition of protein synthesis in the Arabidopsis cell suspension culturea
| Chx concn (μM) | % Incorporation of label ± SD in presence of:
|
|
|---|---|---|
| Chx alone | Chx + 3% sucrose | |
| 0 | 100 ± 8.9b | |
| 10 | 3.1 ± 0.29 | 3.3 ± 0.45 |
| 50 | 2.3 ± 0.10 | 2.3 ± 0.10 |
| 100 | 1.9 ± 0.16 | 2.3 ± 0.16 |
D7 cells were incubated for 24 h in medium without sucrose and then labeled with 35S-labeled amino acids in the presence of Chx and/or sucrose for 6 h. The label incorporated into proteins was determined for triplicate samples by TCA precipitation. Control cells supplied with sucrose alone (0 μM Chx) for 6 h incorporated 48% ± 4.3% of the total intracellular 35S label into protein. This was defined as maximal (100%) incorporation, and other results are presented as a percentage of this level.
Sucrose only.