Figure 4.

Overexpression of NEDD4 promotes ubiquitination and degradation of FOXA1. A, poor expression of FOXA1 in CC suggested on the oncomine database; B-C, protein level of FOXA1 in xenograft tumours (B) and in Lovo and Caco-2 cells determined by western blot analysis; D, mRNA expression of FOXA1 in Lovo and Caco-2 cells measured by RT-qPCR; E, proteome level of FOXA1 in Lovo cells following MG132 administration detected by western blot analysis; F, ubiquitination of FOXA1 in Lovo and Caco-2 cells determined by IP. Data were exhibited as mean ± SD from three independent experiments. In panels B, C and I, data were compared using one-way ANOVA followed by Tukey’s multiple comparison test, while data in panel E ware analysed by the unpaired t test. ** p < 0.01, *** p < 0.001