Table 2.
Primer sequences for RT-qPCR
| Gene | Primer sequence (5ʹ-3ʹ) |
|---|---|
| FOXA1 | F: GCCTTACTCCTACATCTCGCTC |
| R: CTGCTGGTTCTGGCGGTAATAG | |
| NEDD4 | F: GTTATAGATGGGAGCAATGTTGCA |
| R: TCACGCCTTGTTCTCCACTGAG | |
| ATF1 | F: CGCCGAAGACCGCTCCCTCG |
| R: ATCCATGACATCATCGAACTGATC | |
| Vimentin | F: AGGCAAAGCAGGAGTCCACTGA |
| R: ATCTGGCGTTCCAGGGACTCAT | |
| Snail | F: ATCTGCGGCAAGGCGTTTTCCA |
| R: GAGCCCTCAGATTTGACCTGTC | |
| E-cadherin | F: GCCTCCTGAAAAGAGAGTGGAAG |
| R: TGGCAGTGTCTCTCCAAATCCG | |
| miR-340 | F: TTATAAAGCAATGAGACTGATT |
| R: GAACATGTCTGCGTATCTC | |
| GAPDH | F: CGGAGTCAACGGATTTGGTCGTAT |
| R: AGCCTTCTCCATCATTGTGGTGAAGAC | |
| U6 | F: CTCGCTTCGGCAGCACAT |
| R: TTTGCGTGTCATCCTTGCG |
Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; FOXA1, forkhead box A1; NEDD4, neural precursor cell expressed, developmentally down-regulated 4; ATF-1, activating transcription factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.