Figure 6.

Gene expression analysis of PancVax Gel microenvironment. The NanoString nCounter mouse immunology panel was used to analyze gene expression in the tumor bed one week following resection and gel implantation. Results represent five mice in each group. Volcano plots are displayed highlighting (a) the top 40 differentially expressed genes, (b) genes associated with cytokine signaling, (c) genes associated with complement activation and (d) genes associated with innate immunity. (e) A heat map of the top differentially expressed pathways are displayed for each sample within the treatment groups. (f-g) Heat map analysis of (f) phagocytosis and degradation as well as (g) T cell signaling associated gene expression for PancVax gel samples (gray bar) and controls (red bar). (h) Cell type profiling was performed and differences between groups are displayed. All P values were adjusted for false discovery rates using the Benjamini–Yekutieli method