TABLE 2.

Oligonucleotide primers used to amplify a part of the VP4 gene and for the P-typing PCR

Procedure	Primer sequence	P serotype	Locationa
VP4 gene amplification	5′-TTCATTATTGGGACGATTCACA-3′		1064–1085
	5′-CAACCGCAGCGGATATATCATC-3′		1897–1918
P-typing PCRb	5′-TTAAATTCATCTCTTAGTTCTC-3′	P[1]	1505–1526
	5′-GGCCGCATCGGATAAAGAGTCC-3′	P[5]	1704–1725
	5′-TGCCTCATAATATTGTTGGTCT-3′	P[11]	1377–1398

^aLocations of the primers in relation to VP4 gene of BRV strain 223 (14). 

^bThe primers for the P-typing PCR have been described previously (14).