Figure 1.

Cell viability in pCMV-Entry-expressing cells and INS1E-hIAPP-overexpressing cells under ER stress conditions. INS-1E cells were incubated in RPMI 1640 medium supplemented with 2% fetal bovine serum with/without thapsigargin (1 μM) for 6 h or tunicamycin (2 μg/mL) for 16 h and/or with/without 4-PBA (20 μM) at 37 °C with 5% CO₂. Cell viability was analyzed by Cell Counting Kit-8. Values are presented as the mean ± SEM of three experiments.