Figure 7.

The expressions of CHOP, Cu/Zn-SOD, Mn-SOD, catalase, Bcl-2, and Bax proteins in pCMV-Entry-expressing cells and INS1E-hIAPP-overexpressing INS-1E cells under oxidative stress conditions. INS-1E cells were incubated in RPMI 1640 medium supplemented with/without 2% fetal bovine serum and/or nitric oxide (2 mM) or hydrogen peroxide (200 μM) for 24 h at 37 °C with 5% CO₂. CHOP, Cu/Zn-SOD, Mn-SOD, catalase, Bcl-2, and Bax were then analyzed by Western blot (A). The relative amounts of CHOP, Mn-SOD, catalase, and Bcl-2 (B) and Cu/Zn -SOD, and Bax (C) were quantified as described in Materials and Methods. Data represent the mean ± SEM of three experiments. *** p < 0.001 vs. 2% FBS in pCMV-Entry-expressing cells; ^### p < 0.001 vs. 2% FBS in INS1E-hIAPP-overexpressing cells; ^& p < 0.05, ^&& p < 0.01, ^&&& p < 0.001, pCMV-Entry-expressing cells vs. INS1E-hIAPP-overexpressing cells.