Figure 8.

The effect of mitochondrial complex I and complex IV of OXPHOS in pCMV-Entry-expressing cells and INS1E-hIAPP-overexpressing INS-1E cells under oxidative stress conditions. INS-1E cells were incubated in RPMI 1640 medium supplemented with/without 2% fetal bovine serum and/or nitric oxide (2 mM) or hydrogen peroxide (200 μM) for 24 h at 37 °C with 5% CO₂. Mitochondrial complex I and complex IV were then analyzed by Western blot (A). The relative amounts of mitochondrial complex I and complex IV (B) were quantified as described in Materials and Methods. Data represent the mean ± SEM of three experiments. ^& p < 0.05, ^&&& p < 0.001, pCMV-Entry-expressing cells vs. INS1E-hIAPP-overexpressing cells.