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Overview of the study procedure. CRISPR/Cas9-mediated AGAL (α-galactosidase A) knockout (KO) podocytes were compared to wild-type (WT) and lentiviral-mediated rescued (RES) cells via proteome analysis. Expression of dysregulated proteins of interest was further analyzed in endothelial (Ea.hy926), kidney epithelial (HEK293T) cells and patient-specific fibroblasts.
