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. 2021 Oct 27;22(21):11619. doi: 10.3390/ijms222111619

Figure 2.

Figure 2

Senolytic versus senotherapeutic effects of piceatannol. Panel (A): MSCs at 10 DIV were treated for 30 min with 300 μM hydrogen peroxide to induce acute senescence, which was evaluated by beta-gal assay 96 h later. Senescence was also determined in 30 DIV cultures. The pictures show representative images of beta-gal staining in control cultures at 10 DIV (CT), in 10 DIV samples treated with hydrogen peroxide (H), and in replicative senescent cultures at 30 DIV (R). The chart on the right shows the percentage of senescent cells in healthy cells (CT) treated with ABT-737 or PCT (blue bars), in peroxide hydrogen-treated cultures (red bars) and in replicative senescent cultures (green bars). Panel (B): Apoptosis levels were detected in control cultures at 10 DIV (CT), in 10 DIV samples treated with hydrogen peroxide (H), and in replicative senescent cultures at 30 DIV (R). The picture shows the flow cytometry chart of annexin V assay to detect apoptosis. The chart on the right shows the percentage of apoptotic cells in healthy cultures (CT) treated with ABT-737 or PCT (blue bars), in peroxide hydrogen-treated cultures (red bars) and in replicative senescent cultures (green bars). Data are shown with standard deviation (SD) n = 3. The ### p < 0.001 and ## p < 0.01 are the statistical significances between control (CT) and peroxide hydrogen treated samples (H) or replicative senescent cultures (R). The *** p < 0.001 and ** p < 0.01 are statistical significances between control and treated samples.