Figure 2.

PSPAs reduce lung injury and inflammatory responses in mice infected with KP. PSPAs-treated mice and WT mice were infected with 1 × 10⁷ CFU KP for 24 h. (A) The mice lung images and the ratio of wet/dry weight were evaluated. (B) Representative histologic sections of lung tissues from mice of different groups were stained by hematoxylin and eosin (H&E), and a lung injury score was used. (C) The protein concentration in BALF was determined by bicinchoninic acid (BCA) protein quantification. (D) Inflammatory cytokines were measured by ELISA in the BALF of mice in different groups. (E) The number of neutrophils in the blood was counted by routine blood analyses. (F) The MPO activity of the lung was detected by a chemical chromatogram. Data (mean ± SEM) are representative of three independent experiments. One-way ANOVA (Tukey’s post hoc); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.