Table 2.
Method | Target | Super-Resolution Implementation | Fixed/Live-Cell Imaging | Genetically Encoded? | Reference |
---|---|---|---|---|---|
PAINT | Membranes | SMLM, STED | Both | No | [26,117] |
DNA-PAINT | DNA-origami, proteins | SMLM, STED, SOFI | Fixed | No | [30,34,44,118] |
uPAINT | Proteins | SMLM | Live-cell | No | [31] |
RNA-aptamers | RNA | SMLM | Both | Partially 1 | [48] |
FAPs | Proteins | SMLM, STED, SRRF | Both | Partially 1 | [17,61,73,77] |
IRIS 2 | Proteins | SMLM, STED | Both | Both 3 | [8,86,117] |
KECs 4 | Proteins | SMLM | Both | Yes | [21,109] |
Peptide-PAINT | DNA-origami, proteins | SMLM | Fixed | No | [108] |
1 A combination of genetically encoded part with organic fluorogens added externally; 2 includes other methods, based on probe that transiently interacts with a specific target protein; 3 could be used either with organic dyes or fluorescent proteins; 4 and LIVE-PAINT.