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Characterization of BDNF- overexpressing engineered mesenchymal stem cells (eMSCs). (A) Morphology of BDNF-eMSCs. (B) Flow cytometry analyses show that BDNF-eMSCs express specific markers for MSCs such as CD44, CD105, and CD73. N = three per group (C) Cell proliferation rate of BDNF-eMSCs. This result is representative of three independent experiments. (D) BDNF secretion of distinct passages of BDNF-eMSCs measured by ELISA.
