Figure 3.

Structural analysis of carbohydrate surface antigens of K. pneumoniae Kp486 and Kp7De. ¹H NMR spectra of O-antigens isolated from Kp486 (A) and Kp7De (B) compared with the reference spectrum of O1v2 antigen (strain Kp24) (C). ¹H, ¹³C HSQC-DEPT NMR spectra overlaid with ¹H NMR profiles of the polysaccharides isolated from strains Kp486 and Kp7De identified as CPS (D) and LPS O1v2 (E), respectively. NMR spectra were acquired at 298 K. Repeating units of O1v2 (A) and CPS K63 (D) antigen were shown as inset structures. The capital letters refer to carbohydrate residues as described in the inset structures and Table 2 (K63) and Table S2 (O1v2 O-PS).