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The effect of pH on HA activation of different H5N6 viruses. Vero cells infected with (a) rGD/30, (b) rFJ/160, or (c) GD/330-HA-G225W at an MOI of 3 were incubated with pH-adjusted PBS (4.8–6.2). The highest pH at which syncytia formed (arrow) above 50% was defined as the pH threshold.
