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. 2021 Nov 7;10(1):2052–2061. doi: 10.1080/22221751.2021.1997340

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The stability of H5N6 avian influenza viruses. (a) Acid stability of three H5N6 viruses. 10^6.0 EID₅₀ of each virus was diluted in PBS adjusted to the indicated pH and incubated at 37°C for 1 h. Virus titers were then determined in eggs. (b) Heat stability of three H5N6 viruses. 10^6.0 EID₅₀ of each virus was incubated at 50°C for 6 h, and virus titers were then determined in eggs hourly. The statistical analysis was conducted by using multiple t tests with GraphPad Prism 8 software. *, P<0.05 compared with the titers of rGD/330 virus. **, P<0.01 compared with the titers of rGD/330 virus.