Table 2.
Replication and lethality of H5N6 viruses in micea.
| Virus | Virus titer (log10 EID50/mL), mean ± SDb | MLD50 (Log10 EID50) c | ||||
|---|---|---|---|---|---|---|
| Nasal Turbinate | Lung | Spleen | Kidney | Brain | ||
| GD/330 | 5.8 ± 0.4 | 7.1 ± 0.3 | 3.4 ± 0.1 | 3.6 ± 0.1 | 2.5 ± 0.3 | 2.5 |
| rRD-330 | 4.9 ± 0.5 | 7.5 ± 0.0 | 3.3 ± 0.1 | 3.2 ± 0.4 | 2.3 ± 0.5 | 2.5 |
| FJ/160 | 3.1 ± 0.6 | 6.5 ± 0.3 | 1.1 ± 0.5 | - | - | 7.4 |
| rFJ-160 | 2.3 ± 0.5 | 5.4 ± 0.1 | 0.8 ± 0.4 | - | - | 7.4 |
a
Six-week-old female BALB/c mice were used for these studies.
b
Groups of three mice were inoculated intranasally with 106.0 EID50 of the test virus in a 50-µL volume and were killed on day 3 postinoculation (p.i.); organs were then collected for virus titration in eggs. -, no virus was detected in undiluted samples. Virus titers of mice were compared by using the Student-Newman-Keuls test. SD, standard deviation.
c
The 50% mouse lethal dose (MLD50) was determined by intranasally inoculating groups of five mice with 10-fold serial dilutions containing 101.0–106.0 EID50 of virus in a 50-µL volume.