Table 4.
Validation data for the proposed HPLC-PDA method.
| Analyte | tR ± SD (min) | λ (nm) | Linearity | Sensitivity | ||||
|---|---|---|---|---|---|---|---|---|
| Linear Regression | r | Linear Range (μg/mL) | F-Test | LOD (μg/mL) | LOQ (µg/mL) | |||
| NCHA | 3.86 ± 0.02 | 325 | y = 45,336.38x | 0.9992 | 0.37–122.4 | 5072.1 | 0.122 | 0.407 |
| CHA | 6.29 ± 0.02 | 325 | y = 45,621.24x | 0.9993 | 0.32–106.6 | 5430.2 | 0.107 | 0.357 |
| CCHA | 7.29 ± 0.02 | 325 | y = 43,237.18x | 0.9994 | 0.36–120.5 | 5753.8 | 0.121 | 0.403 |
| PB2 | 8.20 ± 0.02 | 280 | y = 14,435.01x | 0.9993 | 0.85–56.6 | 9200.8 | 0.283 | 0.943 |
| ECA | 9.07 ± 0.02 | 280 | y = 12,576.01x | 0.9995 | 0.81–53.7 | 12,206.4 | 0.269 | 0.897 |
| GT | 9.51 ± 0.02 | 285 | y = 4501.262x | 0.9995 | 1.65–551.0 | 13,779.8 | 0.551 | 1.837 |
| CB1 | 10.12 ± 0.02 | 280 | y = 13,183.35x | 0.9998 | 0.89–59.6 | 30,390.9 | 0.298 | 0.993 |
| DGQ | 12.69 ± 0.02 | 350 | y = 25,690.20x | 0.9990 | 0.83–55.4 | 6606.6 | 0.277 | 0.923 |
| HY | 14.52 ± 0.03 | 350 | y = 29,715.40x | 0.9994 | 0.85–56.5 | 10,507.6 | 0.283 | 0.943 |
| MQ | 15.51 ± 0.03 | 350 | y = 32,178.87x | 0.9995 | 0.64–84.8 | 13,826.4 | 0.212 | 0.707 |
| QU | 20.67 ± 0.01 | 370 | y = 62,719.28x | 0.9995 | 0.41–55.3 | 14,002.5 | 0.138 | 0.460 |
tR, retention time; λ, detection wavelength; y, peak area; x, concentration of standard in μg/mL; F-test, value of the statistical Fisher variance ratio for the experimental data. LOD, limit of detection. LOQ, limit of quantification. Analytes: QU, quercetin; MQ, miquelianin; HY, hyperoside; DGQ, wintergreenoside A; ECA, (−)-epicatechin; PB2, procyanidin B2; CB1, cinnamtannin B-1; NCHA, neochlorogenic acid; CHA, chlorogenic acid; CCHA, cryptochlorogenic acid; GT, gaultherin.