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. 2021 Oct 30;22(21):11800. doi: 10.3390/ijms222111800

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Seahorse XF Cell Mito Stress Test: The graphs show representative oxygen consumption rates (OCR) in mitochondrial respiration (D) broken down into maximal respiration (A), spare respiratory capacity (B), and non-mitochondrial oxygen consumption (C). Mitochondrial respiration was measured as OCR in poPSCs cultured for 7 days in the presence of nandrolone or boldenone. The obtained results were compared to the OCR level obtained on poPSCs cultured without the addition of these anabolic steroids (control). Results represent the mean with n = 4 ± standard deviation (SD). Statistical analysis: homogeneity of variance—Levene’s test, normality of distribution—Shapiro–Wilk test, one-way ANOVA, Tukey’s post-hoc test, ** p < 0.01; *** p < 0.001.